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About Raman Spectroscopy

Raman Spectroscopy is a method of chemical analysis used for the analysis of solids, gas and liquids providing a finger print by which the molecule can be identified. Raman analysis is nondestructive, requires very limited sample preparation and allows for sample volumes in the micro liter range. Raman techniques can be used to acquire data through clear plastic and glass vial walls, clear plastic pill packs and clear plastic bags.

Raman Spectroscopy is based on inelastic scattering or Raman scattering of low-intensity, non-destructive laser light. The laser light interacts with the molecular vibrations resulting in a shift in energy providing information about the vibrational modes in the system. No Sample prep is needed and the required amount of tested material could be as low as several picograms or femtoliters. A typical Raman spectrum consists of several narrow bands and is considered a unique signature of the material. Raman spectroscopy shows minimal interference from water which simplifies the analyses of biological fluids and their traces.

The Raman energy shift for a C-H bond is different to that for a C-O bond, and different for a Metal-O bond. By measuring all these various wavelengths of scattered light, one can detect a range of Raman Shift associated with the different bonds and vibrations, for a table of Raman band positions
these advantages and it’s highly spectra specificity allows Raman to be a very powerful tool for sample analysis and chemical monitoring.

Functional Group/ Vibration

 

Region

Raman

υ(C-Br)

 

500 - 700 cm-1

strong

υ(C-Cl)

 

550 - 800 cm-1

strong

υ(C-S) aliphatic

 

630 - 790 cm-1

strong

υ(C-S) aromatic

 

1080 - 1100 cm-1

strong

υ(O-O)

 

845 -900 cm-1

strong

υ(C-O-C)

 

800 -970 cm-1

medium

υ(C-O-C) asym

 

1060 - 1150 cm-1

weak

υ(CC) alicyclic, aliphatic chain vibrations

 

600 - 1300 cm-1

medium

υ(C=S)

 

1000 - 1250 cm-1

strong

υ(C=C) aromatic ring chain vibrations *

 

1580, 1600 cm-1

strong

*Lattice vibrations in crystals, LA modes

 

10 - 200 cm-1

strong

δ(CC) aliphatic chains

 

250 - 400 cm-1

strong

υ(Se-Se)

 

290 -330 cm-1

strong

υ(S-S)

 

430 -550 cm-1

strong

υ(Si-O-Si)

 

450 -550 cm-1

strong

υ(Xmetal-O)

 

150-450 cm-1

strong

υ(C-I)

 

480 - 660 cm-1

strong

δ(CH3)

 

1380 cm-1

medium

δ(CH3) asym

 

1400 - 1470 cm-1

medium

δ(CH2)

 

δ(CH3) asym

 

1400 - 1470 cm-1

strong

υ(C-(NO2))

 

1340 - 1380 cm-1

strong

υ(C-(NO2)) asym

 

1530 - 1590 cm-1

medium

υ(N=N) aromatic

 

1410 - 1440 cm-1

medium

υ(N=N) aliphatic

 

1550 - 1580 cm-1

medium

δ(H2O)

 

~1640 cm-1

weak broad

υ(C=N)

 

1610 - 1680 cm-1

strong

υ(C=C)

 

1500 - 1900 cm-1

strong

υ(C=O)

 

1680 - 1820 cm-1

medium

υ(CC)

 

2100 - 2250 cm-1

strong

υ(CN)

 

2220 - 2255 cm-1

medium

υ(-S-H)

 

2550 - 2600 cm-1

strong

υ(C−Η)

 

2800 - 3000 cm-1

strong

υ(C−Η)

 

3000 - 3100 cm-1

strong

υ((C-H))

 

3300 cm-1

weak

υ(N-H)

 

3300 - 3500 cm-1

medium

υ(O-H)

 

3100 - 3650 cm-1

weak

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